- ￥1180
- BOSTER
- A01064-3-50ul
- 中国
- 2025年07月10日
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- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A01064-3 |
|---|---|
| 产品名称 | Anti-TNFRSF11A Antibody |
| 基因名 | Tnfrsf11a |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 75-80KD |
| 免疫原 | E.coli-derived mouse RANK/Tnfrsf11a recombinant protein (Position: Q30-R203). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Receptor activator of nuclear factor κ B (RANK),also known as TRANCE receptor or TNFRSF11A,is a member of the tumor. It is mapped to 1; 1 E2.1. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins,through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis,autosomal recessive osteopetrosis,and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus. |
| Uniprot ID | Tnfrsf11a: O35305 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-Tnfrsf11a antibody (A01064-3).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat thymus tissue lysates,Lane 2: mouse thymus tissue lysates,Lane 3: mouse RAW264.7 whole cell lysates,Lane 4: mouse SP20 whole cell lysates.Use rabbit anti- Tnfrsf11a 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Tnfrsf11a at approximately 80KD. The expected band size for Tnfrsf11a is at 66KD.|Figure 2.Flow cytometry analysis of HEPA1-6 cell(1x106) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).|Figure 3.Flow cytometry analysis of RH-35 cell(1x106) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).[/list_product_images]
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Anti-RANK/Tnfrsf11a Antibody(A01064-3-50ul)
￥1180
