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- BOSTER
- A00394-50ul
- 中国
- 2025年07月09日
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- 库存：
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A00394 |
|---|---|
| 产品名称 | Anti-GSTP1 Antibody |
| 基因名 | GSTP1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 23KD |
| 免疫原 | E. coli-derived mouse GST3 / GST pi recombinant protein (Position: P2-Q210). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Glutathione S-transferases pi (GSTP1), also known as GST3, is an enzyme that in humans is encoded by the GSTP1 gene. This gene is mapped to 11q13.2. GSTP1 has 7 exons and 6 introns contained within approximately 2.8 kilobases. GSTP1 belongs to Glutathione S-transferases (GSTs) which are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 presents in all tissues and cells, with the exception of red cells, in which only erythrocyte GST(GSTe) is observed. What's more, GSTP1 is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. |
| 研究类别 | 1. Bora PS, Bora NS, Wu XL, Lange LG (Oct 1991). "Molecular cloning, sequencing, and expression of human myocardial fatty acid ethyl ester synthase-III cDNA". J Biol Chem 266 (25): 16774–7.2. Morrow, C. S.; Cowan, K. H.; Goldsmith, M. E. : Structure of the human genomic glutathione S-transferase-pi gene. Gene 75: 3-11, 1989.3. Smith CM, Bora PS, Bora NS, Jones C, Gerhard DS (Nov 1995). "Genetic and radiation-reduced somatic cell hybrid sublocalization of the human GSTP1 gene". Cytogenet Cell Genet 71 (3): 235–9. |
| Uniprot ID | GSTP1: P19157 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of GST3 / GST pi using anti-GST3 / GST pi antibody (A00394). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates, Lane 2: mouse testis tissue lysates,Lane 3: rat spleen tissue lysates,Lane 4: human A549 whole cell lysates,Lane 5: human placenta tissue lysates,Lane 6: human Hela whole cell lysates. -GST3 / GST pi antigen affinity purified polyclonal antibody (Catalog # A00394) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for GST3 / GST pi at approximately 23KD. The expected band size for GST3 / GST pi is at 23KD.|Figure 2. IHC analysis of GST3 / GST pi using anti-GST3 / GST pi antibody (A00394).GST3 / GST pi was detected in paraffin-embedded section of mouse kidney tissue. anti-GST3 / GST pi Antibody (A00394) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. IHC analysis of GST3 / GST pi using anti-GST3 / GST pi antibody (A00394).GST3 / GST pi was detected in paraffin-embedded section of mouse liver tissue. anti-GST3 / GST pi Antibody (A00394) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of GST3 / GST pi using anti-GST3 / GST pi antibody (A00394).GST3 / GST pi was detected in paraffin-embedded section of rat kidney tissue. anti-GST3 / GST pi Antibody (A00394) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IHC analysis of GST3 / GST pi using anti-GST3 / GST pi antibody (A00394).GST3 / GST pi was detected in paraffin-embedded section of rat liver tissue. anti-GST3 / GST pi Antibody (A00394) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. [/list_product_images]
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Anti-GST3/GST pi/Gstp1 Antibody(A00394-50ul)
￥1180
