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- BOSTER
- A00341-50ul
- 中国
- 2025年07月15日
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- 详细信息
- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A00341 |
|---|---|
| 产品名称 | Anti-NGF Antibody |
| 基因名 | NGF |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 45KD/75KD |
| 免疫原 | E. coli-derived human NGF/NGF Beta recombinant protein (Position: S122-A241). Human NGF/NGF Beta shares 92.4% amino acid (aa) sequence identity with rat NGF/NGF Beta. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Nerve growth factor is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. The nucleotide sequence of human and mouse beta-NGF are very similar. The beta-subunits of nerve growth factor (NGFB) have been assigned to mouse chromosome 3 and human chromosome 1p22. The human gene for the beta subunit of nerve growth factor is located on the proximal short arm of chromosome 1. A mutation in the nerve growth factor beta gene (NGFB) causes loss of pain perception. |
| 研究类别 | 1. Dracopoli, N. C.; Rose, E.; Whitfield, G. K.; Guidon, P. T.; Bale, S. J.; Chance, P. A.; Kourides, I. A.; Housman, D. E. : Two thyroid hormone regulated genes, the beta-subunits of nerve growth factor (NGFB) and thyroid stimulating hormone (TSHB), are located less than 310 kb apart in both human and mouse genomes. Genomics 3: 161-167, 1988. 2. Einarsdottir, E.; Carlsson, A.; Minde, J.; Toolanen, G.; Svensson, O.; Solders, G.; Holmgren, G.; Holmberg, D.; Holmberg, M. : A mutation in the nerve growth factor beta gene (NGFB) causes loss of pain perception. Hum. Molec. Genet. 13: 799-805, 2004.3. Francke, U.; de Martinville, B.; Coussens, L.; Ullrich, A. : The human gene for the beta subunit of nerve growth factor is located on the proximal short arm of chromosome 1. Science 222: 1248-1251, 1983 |
| Uniprot ID | NGF: P01138 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| (ELISA)： | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of NGF/NGF Beta using anti-NGF/NGF Beta antibody (A00341). Lane 1: Recombinant Human NGF/NGF Beta Protein 0.5ng -NGF/NGF Beta antigen affinity purified polyclonal antibody (Catalog # A00341) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for NGF/NGF Beta at approximately 13KD. The expected band size for NGF/NGF Beta is at 13KD[/list_product_images]
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Anti-NGF/NGF Beta Antibody(A00341-50ul)
￥1180
