- ￥1180
- BOSTER
- A00066-1-50ul
- 中国
- 2025年07月10日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A00066-1 |
|---|---|
| 产品名称 | Anti-HMGB1 Antibody |
| 基因名 | HMGB1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 25KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human HMGB1 (124-154aa DVAKKLGEMWNNTAADDKQPYEKKAAKLKEK), identical to the related mouse and rat sequences. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | High mobility group box 1 protein, also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a protein that in humans is encoded by the HMGB1 gene. This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. |
| 研究类别 | 1. Ferrari S, Finelli P, Rocchi M, Bianchi ME (July 1996). "The active gene that encodes human high mobility group 1 protein (HMG1) contains introns and maps to chromosome 13". Genomics. 35 (2): 367–71.2. Klune JR, Dhupar R, Cardinal J, Billiar TR, Tsung A (2008)."HMGB1: endogenous danger signaling". Mol. Med. 14 (7-8): 476–84.3. Yang H, Hreggvidsdottir HS, Palmblad K, Wang H, Ochani M, Li J, Lu B, Chavan S, Rosas-Ballina M, Al-Abed Y, Akira S, Bierhaus A, Erlandsson-Harris H, Andersson U, Tracey KJ (June 2010). "A critical cysteine is required for HMGB1 binding to Toll-like receptor 4 and activation of macrophage cytokine release". Proc. Natl. Acad. Sci. U.S.A. 107 (26): 11942–7. |
| Uniprot ID | HMGB1: P09429 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- HMGB1 antibody (A00066-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
Use rabbit anti- HMGB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HMGB1 at approximately 25KD. The expected band size for HMGB1 is at 25KD.|Figure 2. IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).HMGB1 was detected in paraffin-embedded section of mouse intestine tissues. anti-HMGB1 Antibody (A00066-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).HMGB1 was detected in paraffin-embedded section of mouse liver tissues. anti-HMGB1 Antibody (A00066-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).HMGB1 was detected in paraffin-embedded section of rat intestine tissues. anti-HMGB1 Antibody (A00066-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).HMGB1 was detected in paraffin-embedded section of rat liver tissues. anti-HMGB1 Antibody (A00066-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 6. IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).HMGB1 was detected in paraffin-embedded section of human mammary cancer tissues. anti-HMGB1 Antibody (A00066-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 7. IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).HMGB1 was detected in paraffin-embedded section of human placenta tissues. anti-HMGB1 Antibody (A00066-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 8. Immunofluorescence assay analysis of U2OS cell at a dilution of 1@50 dylight 550 goat anti-rabbit IgG(red) blue Pseudocolor blue=DAPI|Figure 9. Immunofluorescence assay analysis of A431 cell at a dilution of 1@50 dylight 550 goat anti-rabbit IgG(red) blue Pseudocolor blue=DAPI|Figure 10. Flow cytometry analysis of THP-1 cell HELA(1x106) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488 . Unlabelled sample (Red line)[/list_product_images]
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
Use rabbit anti- HMGB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HMGB1 at approximately 25KD. The expected band size for HMGB1 is at 25KD.|Figure 2. IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).HMGB1 was detected in paraffin-embedded section of mouse intestine tissues. anti-HMGB1 Antibody (A00066-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).HMGB1 was detected in paraffin-embedded section of mouse liver tissues. anti-HMGB1 Antibody (A00066-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).HMGB1 was detected in paraffin-embedded section of rat intestine tissues. anti-HMGB1 Antibody (A00066-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).HMGB1 was detected in paraffin-embedded section of rat liver tissues. anti-HMGB1 Antibody (A00066-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 6. IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).HMGB1 was detected in paraffin-embedded section of human mammary cancer tissues. anti-HMGB1 Antibody (A00066-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 7. IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).HMGB1 was detected in paraffin-embedded section of human placenta tissues. anti-HMGB1 Antibody (A00066-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 8. Immunofluorescence assay analysis of U2OS cell at a dilution of 1@50 dylight 550 goat anti-rabbit IgG(red) blue Pseudocolor blue=DAPI|Figure 9. Immunofluorescence assay analysis of A431 cell at a dilution of 1@50 dylight 550 goat anti-rabbit IgG(red) blue Pseudocolor blue=DAPI|Figure 10. Flow cytometry analysis of THP-1 cell HELA(1x106) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488 . Unlabelled sample (Red line)[/list_product_images]
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Anti-HMGB1 Antibody(A00066-1-50ul)
￥1180
