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- BOSTER
- A00023-50ul
- 中国
- 2025年07月12日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A00023 |
|---|---|
| 产品名称 | Anti-EGFR Antibody |
| 基因名 | EGFR |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 175KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human EGFR (25-57aa LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNN), different from the related mouse sequence by two amino acids. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed. |
| 研究类别 | 1. Herbst RS (2004). "Review of epidermal growth factor receptor biology". Int. J. Radiat. Oncol. Biol. Phys. 59 (2 Suppl): 21–6.2. Kuan CT, Wikstrand CJ, Bigner DD (June 2001). "EGF mutant receptor vIII as a molecular target in cancer therapy". Endocr. Relat. Cancer 8 (2): 83–96.3. Wang, K.; Yamamoto, H.; Chin, J. R.; Werb, Z.; Vu, T. H.: Epidermal growth factor receptor-deficient mice have delayed primary endochondral ossification because of defective osteoclast recruitment. J. Biol. Chem. 279: 53848-53856, 2004. |
| Uniprot ID | EGFR: P00533 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- EGFR antibody (A00023). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: A431 whole cell lysates,
Lane 2: A431 whole cell lysates.
Use rabbit anti- EGFR 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for EGFR at approximately 175KD. The expected band size for EGFR is at 134KD.|Figure 2. Flow Cytometry analysis of A431 cells using anti- EGFR antibody (A00023).
Overlay histogram showing A431 cells stained with A00023 (Blue line).. And then incubated with rabbit anti-EGFR Antibody (A00023,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 3. IHC analysis of EGFR using anti-EGFR antibody (A00023).
EGFR was detected in paraffin-embedded section of human glioma tissues. anti-EGFR Antibody (A00023) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis of EGFR using anti-EGFR antibody (A00023).
EGFR was detected in paraffin-embedded section of human placenta tissues. anti-EGFR Antibody (A00023) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.[/list_product_images]
Lane 1: A431 whole cell lysates,
Lane 2: A431 whole cell lysates.
Use rabbit anti- EGFR 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for EGFR at approximately 175KD. The expected band size for EGFR is at 134KD.|Figure 2. Flow Cytometry analysis of A431 cells using anti- EGFR antibody (A00023).
Overlay histogram showing A431 cells stained with A00023 (Blue line).. And then incubated with rabbit anti-EGFR Antibody (A00023,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 3. IHC analysis of EGFR using anti-EGFR antibody (A00023).
EGFR was detected in paraffin-embedded section of human glioma tissues. anti-EGFR Antibody (A00023) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis of EGFR using anti-EGFR antibody (A00023).
EGFR was detected in paraffin-embedded section of human placenta tissues. anti-EGFR Antibody (A00023) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.[/list_product_images]
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Anti-EGFR Antibody(A00023-50ul)
￥1180
