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- BOSTER
- A00004-50ul
- 中国
- 2025年07月09日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A00004 |
|---|---|
| 产品名称 | Anti-Catenin-β Antibody |
| 基因名 | CTNNB1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 95KD |
| 免疫原 | E. coli-derived human beta Catenin recombinant protein (Position: A2-K233). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer. |
| 研究类别 | 1. N. Peyrieras, D. Louvard and F. Jacob. "Characterization of antigens recognized by monoclonal and polyclonal antibodies directed against uvomorulin" in Proceedings of the National Academy of Sciences of the United States of America (1985) Volume 82, pages 8067-8071.2. Thompson MD, Monga SP (2007). "WNT/beta-catenin signaling in liver health and disease". Hepatology 45 (5): 1298–305. |
| Uniprot ID | CTNNB1: P35222 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-100 |
| Immunohistochemistry in frozen section (IHC-F): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- Catenin-β antibody (A00004). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: MCF-7 whole cell lysates,
Lane 3: A431 whole cell lysates,
Lane 4: RT4 whole cell lysates,
Lane 5: SIHA whole cell lysates,
Lane 6: T47D whole cell lysates,
Lane 7: rat brain tissue lysates,
Lane 8: rat liver tissue lysates,
Lane 9: mouse brain tissue lysates,
Lane 10: mouse liver tissue lysates.
Use rabbit anti- Catenin-β 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Catenin-β at approximately 95KD. The expected band size for Catenin-β is at 85KD.|Figure 2. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of human liver cancer tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of human mammary cancer tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of human prostatic cancer tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of human tonsil tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 6. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of mouse intestine tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 7. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of mouse heart tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 8. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of mouse liver tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 9. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of rat heart tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 10. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of rat intestine tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 11. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of rat liver tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 12. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of rat spleen tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 13. Flow Cytometry analysis of A549 cells using anti-CTNNB1 antibody (A00004).Overlay histogram showing A549 cells stained with A00004 (Blue line).. And then incubated with rabbit anti-CTNNB1 Antibody (A00004,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 14. IF analysis of CTNNB1 using anti- CTNNB1 antibody (A00004) CTNNB1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- CTNNB1 Antibody (A00004) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 15. IF analysis using anti- Catenin-β antibody (A00004). detected in paraffin-embedded section of mouse liver cancer tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1032) and counterstained with DAPI (blue). [/list_product_images]
Lane 1: Hela whole cell lysates,
Lane 2: MCF-7 whole cell lysates,
Lane 3: A431 whole cell lysates,
Lane 4: RT4 whole cell lysates,
Lane 5: SIHA whole cell lysates,
Lane 6: T47D whole cell lysates,
Lane 7: rat brain tissue lysates,
Lane 8: rat liver tissue lysates,
Lane 9: mouse brain tissue lysates,
Lane 10: mouse liver tissue lysates.
Use rabbit anti- Catenin-β 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Catenin-β at approximately 95KD. The expected band size for Catenin-β is at 85KD.|Figure 2. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of human liver cancer tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of human mammary cancer tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of human prostatic cancer tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of human tonsil tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 6. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of mouse intestine tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 7. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of mouse heart tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 8. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of mouse liver tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 9. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of rat heart tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 10. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of rat intestine tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 11. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of rat liver tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 12. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004).CTNNB1 was detected in paraffin-embedded section of rat spleen tissue. anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 13. Flow Cytometry analysis of A549 cells using anti-CTNNB1 antibody (A00004).Overlay histogram showing A549 cells stained with A00004 (Blue line).. And then incubated with rabbit anti-CTNNB1 Antibody (A00004,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 14. IF analysis of CTNNB1 using anti- CTNNB1 antibody (A00004) CTNNB1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- CTNNB1 Antibody (A00004) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 15. IF analysis using anti- Catenin-β antibody (A00004). detected in paraffin-embedded section of mouse liver cancer tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1032) and counterstained with DAPI (blue). [/list_product_images]
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Anti-beta Catenin/CTNNB1 Antibody(A00004-50ul)
￥1180
