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G-292, clone A141B1
1×10^6 cells
骨肉瘤
人
上皮细胞样
是
骨
复苏细胞常温/冻存管干冰
贴壁生长
T25培养瓶/冻存管
Product category | Human cells |
Synonyms | G 292 Clone A 141B1; G-292, clone A141B1; G292CLONEA141B1; G-292; G292 |
Organism | Homo sapiens, human |
Cell type | - |
Morphology | fibroblast |
Tissue | Bone |
Disease | Osteosarcoma |
Applications | 3D cell culture |
Growth properties | Adherent |
Derivation | G-292, clone A141B1 was derived from a pediatric tumor. |
Population doubling time | - |
Age | 9 years |
Ethnicity | White |
Gender | Female |
Expression markers | Epidermal growth factor (EGF) |
Isoenzymes | G6PD, B |
Unpacking and storage instructions |
|
Complete medium | The base medium for this cell line is formulated McCoy's 5a Medium Modified. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
Temperature | 37°C |
Atmosphere | 95% Air, 5% CO2 |
Handling procedure | To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
|
Subculturing procedure | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal: Every 2 to 3 days. |
Reagents for cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO. |
Mycoplasma contamination | Not detected |
STR profiling | Amelogenin:X CSF1PO:11,12 D2S1338:17 D3S1358:16,17 D5S818:12,13 D7S820:9 D8S1179:15 D13S317:9,14 D16S539:12 D18S51:18 D19S433:16.2 D21S11:31 FGA:22 Penta D:13 Penta E:17 TH01:6 TPOX:8 vWA :14 |
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