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Clone M-3(S91)
1×10^6 cells
黑素瘤
人
上皮细胞样
是
复苏细胞常温/冻存管干冰
贴壁生长
T25培养瓶/冻存管
Product category | Animal cells |
Synonyms | Cloudman S91 melanoma clone M-3; S91 clone M3; CLONE M3; M3 Clone M-3; Cloudman M3 |
Organism | Mus musculus, mouse |
Cell type | melanocyte |
Morphology | epithelial |
Tissue | Skin |
Disease | Melanoma |
Applications | 3D cell culture |
Growth properties | Adherent |
Derivation | Clone M-3, a melanin-producing cell line was adapted to cell culture by Y. Yasumura, A.H. Tashjian and G. Sato from a Cloudman S91 melanoma in a (C X DBA) F1 male mouse obtained from the Jackson Memorial Laboratory, Bar Harbor, Maine. |
Population doubling time | - |
Age | - |
Ethnicity | - |
Strain | DBA |
Gender | Male |
Karyotype | Stemline number is hypertetraploid. Karyotype is stable within stemline number. Marker chromosomes: A medium size chromosome with a submedian centromere and a smaller chromosome with a median centromere., The remaining 81 chromosomes have terminal centromeres, the first one is larger than normal. A minute chromosome was noted in 20% of the cells. |
Isoenzymes | G6PD, A |
Tumorigenic | Yes; Yes, in C57BL mice |
Virus susceptibility | Herpes simplex virus Vaccinia virus Vesicular stomatitis, Glasgow (Indiana) Vesicular stomatitis, Orsay (Indiana) |
Genes expressed | melanin |
Comments | Tested and found negative for ectromelia virus (mousepox). |
Unpacking and storage instructions |
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Complete medium | The base medium for this cell line is formulated F-12K Medium. To make the complete growth medium, add the following components to the base medium:
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Temperature | 37°C |
Atmosphere | 95% Air, 5% CO2 |
Handling procedure | To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
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Subculturing procedure | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal: Every other day |
Reagents for cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO. |
Mycoplasma contamination | Not detected |
STR profiling | - |
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