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CCD-1069Sk
1×10^6 cells
人
上皮细胞样
是
复苏细胞常温/冻存管干冰
贴壁生长
T25培养瓶/冻存管
Product category | Human cells |
Organism | Homo sapiens, human |
Cell type | fibroblast |
Morphology | fibroblast |
Tissue | Skin |
Disease | Carcinoma |
Applications | 3D cell culture |
Growth properties | Adherent |
Derivation | The line was established from skin taken from normal breast tissue removed at mastectomy for invasive ductal carcinoma. |
Population doubling time | - |
Age | 70 years |
Ethnicity | - |
Gender | Female |
Karyotype | - |
Isoenzymes | - |
Expression markers | - |
Comments | The line was established from skin taken from normal breast tissue removed at mastectomy for invasive ductal carcinoma. Cells senesce after approximately 23 population doublings. |
Unpacking and storage instructions |
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Complete medium | The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
Temperature | 37°C |
Atmosphere | 95% Air, 5% CO2 |
Handling procedure | To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at –70°C. Storage at –70°C will result in loss of viability.
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Subculturing procedure | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
Reagents for cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO (ATCC 4-X) |
Mycoplasma contamination | Not detected |
STR profiling | Amelogenin: X CSF1PO: 10,12 D13S317: 8,11 D16S539: 12,13 D5S818: 9,11 D7S820: 9,10 TH01: 6,9 TPOX: 8 vWA: 16,18 D3S1358: 16,17 D21S11: 29,31 D18S51: 14,15 Penta_E: 5,15 Penta_D: 11,12 D8S1179: 14 FGA: 19,21 D19S433: 15 D2S1338: 17,20 |
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