E3 ligase Ligand-Linker Conjug

Pulse-Chase Experiment for the Analysis of Protein Stability in Cultured Mammalian Cells by Covalent Fluorescent Labeling of Fusion Proteins

by fluorescent image analysis. Herein, we demonstrated that the method allows analysis of the intracellular protein stability as regulated by protein-degradation signals or an exogenously expressed E3 ubiquitin ligase.

Receptor-Directed Molecular Conjugates for Gene Transfer

), and in vivo (27 –40 ). This method has potential for human gene therapy, once it is perfected in animal models. DNA, noncovalently bound to a poly cation polymer that is chemically conjugated to a ligand, can be bound to the cell surface and internalized

Studies of the Ubiquitin Proteasome System

modification; NEDD8; E1; E2; E3