- ￥3680
- BOSTER
- BA1012-1ml
- 中国
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
1ml
Basic Information
| Product Name | Alkaline Phosphatase Conjugated AffiniPure Rabbit Anti-rat IgG (H+L) |
|---|---|
| Catalog Number | BA1012 |
| Size | 100μl |
| Concentration | 1mg/ml |
| Ig Type | IgG |
| Immunogen | Rat IgG (whole molecular) |
| Host | Rabbit |
| Purity | The antibody was purified from antisera by immunoaffinity chromatography. |
| Conjugation | Alkaline Phosphatase |
| Form | Concentrated, Liquid |
| Tested Application | WB,ELISA |
| Contents | 1mg/ml of Alkaline Phosphatase conjugated specific antibody;0.05M Tris buffer, pH8.0, containing 1% BSA, 1mM MgCl2, 50% glycerol, with 15mM sodium azide as a preservative. |
| Suggested Working Concentration | Western Blotting: 1:3000-10000 (ECL)Western Blotting: 1:300-1500(DAB detection)Direct ELISA: 1:5000-30000(TMB detection)Optimal working dilutions must be determined by end users. |
Application Notes
Diluted by neutral PBS or TBS.
Storage
At -20℃ for 12 months from the date of receipt.
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文献和实验ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
9. Wash in buffer. 3 minutes. 10. Incubate in rabbit anti-mouse immunoglobins. 30 minutes. 11. Jet wash in buffer. 12. Wash in buffer. 3 minutes. 13. Incubate in alkaline phosphatase-mouse anti-alkaline phosphatase 30 minutes. 14. Jet
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
dehydrogenases. Progr. Histochem. Cytochem . 4 :225. Berger, J., A.D. Howard, L. Gerber, B.R. Cullen, and S. Udenfriend. 1987. Expression of active, membrane-bound human placental alkaline phosphatase by transfected simian cells. Proc. Natl. Acad. Sci
and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
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