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- 2025年07月08日
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文献和实验Ammonium Sulfate Binding Assay for Cells
pellet again with cold-room microfuge. Aspirate out supernatant. 5. Resuspend in 100-150 μl 1% SDS or 0.1% formic acid. Transfer into counting vials and add 3 ml scintillation fluid. Count.
Antibody Purification: Ammonium Sulfate Fractionation or Gel Filtration
Antibodies can be purified by a variety of methods based on their unique physical and chemical properties such as size, solubility, charge, hydrophobicity and binding affinity. This chapter focuses on ammonium sulfate precipitation
Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration
In this chapter, two commonly used techniques that are utilized in many immunoglobulin purification schemes are described. The first procedure, ammonium sulfate fractionation, is generally employed as the initial step in the isolation of crude
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