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文献和实验Assays for the Interferon-Induced Enzyme 2,5 Oligoadenylate Synthetases
Inhibition of protein synthesis by interferon treatment is mediated by two major pathways: the 2′–5′-linked oligoadenylates [2–5 (A)] synthetase-RNase L pathway and the double-stranded ribonucleic acid-dependent protein kinase-mediated
to induce photochemical reactions that result in covalent linkages between the nucleic acid and the protein. The protein can be a purified or a recombinant RNA binding protein, but perhaps a more common application is the identification of proteins
Protein Engineering of Interferon Alphas
differences in these proteins may be responsible for a significant variety of biological actions. Understanding the structure and function of human IFN-α is very important. Recombinant techniques are important tools for the production and modification of IFN
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