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文献和实验depends on product and its function. N-terminal sequencing revealed that the purified rBoNTC(Hc )-N is missing the first eight amino acids of the N-terminus of the protein, whereas the purified rBoNTC(Hc )-C protein is intact. After a mouse bioassay test
°C for several hrs or at 4°C overnight. Completely aspirate collagen from the dish before adding cells. Note: Do not allow Vitrogen stock to warm, or it will begin to gel. Culture mouse keratinocytes at 33-34°C, 8% CO2
Mouse Strains and Genetic Nomenclature
. We also provide some guidance to make the best possible choice when establishing an experimental protocol. Curr. Protoc. Mouse Biol. 1:213?238. © 2011 by John Wiley & Sons, Inc. Keywords: inbred strains; congenic strains; recombinant inbred
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