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文献和实验Human Papillomavirus Detection by PCR and Typing by Dot-Blot
and (2) late-expressing genes, and (3) a noncoding upstream regulatory region (URR) (Fig. 1 ). The early region is downstream of the URR and consist of six open reading frames (ORFs) (El, E2, E4, E5, E6, and E7). El encodes a DNA binding protein involved
Papillomavirus DNA Replication
Papillomavirus genomes replicate and are maintained as stable extrachromosomal plasmid DNA (episomes) in many cell lines (reviewed in ref. 1 ). This process requires the viral E1 and E2 proteins and the origin
Purification and Characterization of Legionella U-Box-Type E3 Ubiquitin Ligase
LubX is one such bacterial E3 ubiquitin ligase. We describe here the method to purify soluble LubX protein using GST-tag and Escherichia coli overexpression systems. Using the purified protein together with recombinant ubiquitin, E1, and E2 enzymes
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