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- 2025年07月13日
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文献和实验Introduction to the EMSA (Gel Shift) Technique
The interaction of proteins with DNA is central to the control of many cellular processes including DNA replication, recombination and repair, transcription, and viral assembly. One technique that is central to studying gene regulation
Western Blotting with Biotinylated Antibodies
Western Blotting with Biotinylated Antibodies SAMPLE PREPARATION For Protein Concentration Determination of Cell Culture Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline
NICK TRANSLATION OF dsDNA WITH BIOTINYLATED OR DIGOXIGENIN dUTP
NICK TRANSLATION OF dsDNA WITH BIOTINYLATED OR DIGOXIGENIN dUTP For the use with FISH (fluorescent in situ hybridization) we always label entire plasmid DNA and do not cut out the insert. 1. mix together x µl
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