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- 2025年07月14日
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文献和实验Expression of Microbial Phytases in Yeast Systems and Characterization of the Recombinant Enzymes
the transformants. Partial purification, biochemical characterization, and in vitro function assay of the recombinant phytases are also discussed. Key Words: Aspergillus niger ; electroporation; environment; Escherichia coli ; food; feed; gene expression
Construction of Adenovirus Type 5 Early Region 1 and 4 Virus Mutants
, and viral progeny are isolated and amplified. The advantages of this strategy are multiple: all cloning steps are carried out in Escherichia coli , and any genetic region of the viral E1 and/or E4 transcription units can be specifically modified or deleted
Purification and Characterization of Legionella U-Box-Type E3 Ubiquitin Ligase
LubX is one such bacterial E3 ubiquitin ligase. We describe here the method to purify soluble LubX protein using GST-tag and Escherichia coli overexpression systems. Using the purified protein together with recombinant ubiquitin, E1, and E2 enzymes
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