Recombinant Saccharomyces cere
visiae DNA repair and recombination protein RAD52 (RAD52), partial- 询价
- Biomatik
- RPC22774
- 2025年07月13日
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文献和实验In Vitro Monitoring of Base Excision Repair in Saccharomyces cerevisiae
of base damage and of individual mechanistic steps within BER. We used these protocols to investigate a new role of the DNA double strand break repair protein XRS1 in BER (1).
FRET-Based Assays to Monitor DNA Binding and Annealing by Rad52 Recombination Mediator Protein
a robust signal, which reports on the association of a recombination mediator with its substrate and on the state of substrate DNA within the complex. Eukaryotic Rad52 protein mediates recombination, repair, and restart of collapsed replication forks
Chromatin immunoprecipitation protocol for Saccharomyces cerevisiae
is usually repaired by recombination. However, in this strain the donor loci for repair were deleted in order to prevent repair by recombination. This allows us to monitor protein binding at the HO-induced DSB. (comment 1) Inoculate YPAD medium
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