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文献和实验Expression of Recombinant Cytochromes c in E. coli
for proper folding and stability. However, significant advances in expression of recombinant cytochromes c have been made during the last decade. It has been shown that a single gene cluster, ccmA–H , is responsible for cytochrome c maturation in Escherichia
Strategies to Optimize Protein Expression in E. coli
. 5:478‐487. Baneyx, F. and Mujacic, M. 2004. Recombinant protein folding and misfolding in Escherichia coli. Nat. Biotechnol. 22:1399‐1408
A Dual-Expression Vector Allowing Expression in E. coli and P. pastoris, Including New Modifications
Heterologous gene expression is often treated empirically and a number of host organisms are systematically tested. Early successes in the expression of recombinant proteins were achieved using the well-studied bacterium Escherichia coli
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