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文献和实验Experimental Applications: Human Acetylcholinesterase as a Model Nervous System Protein
A DNA sequence encoding the brain and muscle form of human AChE (AChE-T) was constructed in our laboratory from cloned cDNA and genomic sequences, and tentatively identified by its homology to known ChEs (Soreq et al., 1990). This putative
Introduction of human 14-3-3 proteins
acid sequence of human 14-3-3 protein eta was highly homologous to that of previously reported bovine and rat 14-3-3 proteins with only two amino acid differences.The sequence carries structural features as putative regions responsible for activation
Methods to Detect Protein Glutathionylation
irreversible oxidative modification. Thus, determining the ability of a given protein to become glutathionylated can provide insight into its redox regulation and putative role in dictating cellular response to oxidative and nitrosative stress. Methods
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