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- 2025年07月16日
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文献和实验Eukaryotic Cell Labeling and Preparation for 2-D
Two-dimensional polyacrylamide gel electrophoresis (2-DE) provides the ability to resolve and quantify thousands of proteins from an unfractionated eukaryotic cell lysate. The use of 2-DE in studying eukaryotic cells has run the gambit
DNA TRANSFECTION OF EUKARYOTIC CELLS USING CALCIUM PHOSPHATE
.05 with NaOH Autoclave Store frozen in aliquots 3. 1 X HEBS/15% glycerol. 50ml 2 x HEBS ) 15ml glycerol ) 35ml DDW ) Autoclave and store at -20¡ in aliquots 4. Carrier DNA. Mouse liver DNA, sheared at 90ug/ml in 0.2XSSC
A Protocol for Heterologous Expression and Functional Assay for Mouse Pheromone Receptors
. We have demonstrated that calreticulin, a housekeeping chaperone commonly expressed in most eukaryotic cells, is sparsely expressed in the vomeronasal sensory neurons (VSNs). Stable knock down of calreticulin in a HEK293T derived cell line (R24 cells) allows
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