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文献和实验Assays for Cyclin-Dependent Kinase Inhibitors
Cyclin-dependent kinases (CDK) are serine/threonine kinases that regulate cell cycle control and progression (1 ,2 ). A CDK holoenzyme complex is active if associated with its cyclin partner and if the complex is phosphorylated at specific
Evaluation of Alterations in the Tumor Suppressor Genes INK4A and INK4B in Human Bladder Tumors
The progression through the cell cycle is monitored by positive and negative regulators. One family of negative regulators has been reported to act as cyclin-dependent kinase inhibitors (CKI ) (1 –3 ); and these, in turn
Introduction of human 14-3-3 proteins
of mRNA encoding human 14-3-3 protein,a protein kinase-dependent activator of tyrosine and tryptophan hydroxylases and an endogenous inhibitor of protein kinase C.The 1,730-nucleotide sequence of the cloned cDNA contains 191 bp of a 5'-noncoding region
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