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文献和实验labeled above. The controls used in this experiment were the Pum- (bop-) host strain SD23, strain 100E (SD23 containing plasmid with unmutated promoter) and TATA box mutants (1B2, 2B12, 2H10 and 1D2). A sequencing reaction (C lane) performed
Promoter analysis by saturation muta
in this experiment were the Pum- (bop -) host strain SD23, strain 100E (SD23 containing plasmid with unmutated promoter) and TATA box mutants (1B2, 2B12, 2H10 and 1D2). A sequencing reaction (C lane) performed with the same primer on pMS1 template (plasmid containing
(dimensions gram per second) which depends on both the size and shape of the protein. For a given mass of protein (or given volume), f will increase as the protein becomes elongated or asymmetrical (f can be replaced by an equivalent expression containing R s
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