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- 2025年07月13日
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文献和实验Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
are produced as soluble recombinant proteins by transiently transfecting a mammalian cell line. The proteins are purified using an oligo‐His tag using a bespoke purification apparatus (the “Protein Press”) and subsequently printed onto glass slides
Strategies to Optimize Protein Expression in E. coli
Abstract Table of Contents Figures Literature Cited Abstract Recombinant protein expression
Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
, several methods have been developed to analyze protein?protein interactions under native conditions. One of them, tandem affinity purification (TAP), combines two affinity?purification steps to allow isolation of high?purity protein complexes. This unit presents
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