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- 2025年07月15日
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文献和实验Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
protein discovery initiative (SPDI), a large‐scale effort to identify novel human secreted and transmembrane proteins: A bioinformatics assessment. Genome Res. 13:2265‐2270.
Synchronizing Protein Transport in the Secretory Pathway
in . The FUT8 gene was inserted between Asc I and Eco RI sites, and since the alpha‐(1, 6)‐fucosyltransferase is a type II transmembrane protein, the retention occurs in the lumen using Str‐KDEL as a hook. HeLa cells were transiently transfected with a plasmid
Site‐Specific Protein Labeling with SNAP‐Tags
. Griffin, B.A., Adams, S.R., and Tsien, R.Y. 1998. Specific covalent labeling of recombinant protein molecules inside live cells. Science 281:269–272.
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