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- 2025年07月12日
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文献和实验Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation
Fibromodulin Lumican PRELP (proline/arginine-rich and leucine-rich repeat protein) Chondroadherin
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
are produced as soluble recombinant proteins by transiently transfecting a mammalian cell line. The proteins are purified using an oligo‐His tag using a bespoke purification apparatus (the “Protein Press”) and subsequently printed onto glass slides
with a transmembrane anchor, but after translocation across the membrane of the endoplasmic reticulum, the ecto-domain of the protein is cleaved and covalently linked to a preformed GPI anchor by a specific transamidase enzyme. At least one human disease, paroxysmal
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