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文献和实验High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
.1 ) Phosphate‐buffered saline (PBS; Fisher Scientific) Alexa Fluor 546–conjugated goat anti—rabbit IgG (Invitrogen, cat. no. A‐11035
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with 1 g/ml OVA peptide (residues 323–339) in RPMI medium containing 10% FBS. Transfection For electroporations, 2.5 mol dsRNA and/or 20 g of pEGFP-N3 plasmid (Clontech Laboratories, Palo Alto, CA) were added to prechilled 0.4-cm electrode gap
Antigen Design Sera Purification Tech Sheet
against peptides conjugated to BSA will also contain antibodies to BSA, false positives may result. Although KLH is large and immunogenic, it may precipitate during cross-linking, making it difficult to handle in some cases. Ovalbumin (OVA) is another useful
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