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- Biomatik
- RPU43859
- 2025年07月08日
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文献和实验Performing In Vitro Sumoylation Reactions Using Recombinant Enzymes
for in vitro sumoylation of RanGAP1 and Sp100 in the absence or presence of the SUMO E3 ligase RanBP2, respectively, are provided. Both protocols can be adapted easily to test in vitro conjugation of other target proteins and/or E3 ligases.
Isolation of Recombinant DNA Elongation Proteins
This chapter summarizes isolation procedures of four recombinant human proteins crucial for DNA replication: (a) the replicative DNA polymerase (pol) δ, (b) proliferating cell nuclear antigen (PCNA), (c) replication protein A (RP-A), and (d
Cloning of Variable Domains from Mouse Hybridoma by PCR
propagation in E. coli or other recombinant production systems. Further, monoclonal antibodies with promising specificity, but low affinity, can be improved by phage display, or mouse antibodies can be humanised for therapy. This chapter describes sets
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