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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
-Phos staining. Background staining can be due to endogenous alkaline phosphatase activities or reduction of NBT from other sources (e.g. NADPH). It is also enhanced by light. The most effective inhibitor is heat. To reduce background
Endothelial Cell Systems - Instructions For Use
Extract (BBE) supplement. EGM® is a modified MCDB 131 formulation and is supplied fully supplemented with the following: (amounts indicate final concentration, except BBE) 10 ng/ml hEGF (human recombinant Epidermal Growth Factor) 1.0 µg/ml
Strategies to Optimize Protein Expression in E. coli
., Wang, Y., Ma, J.B., Gao, D.M., Wu, H.M., and Chen, H.B. 2006. Over‐expression and purification of isotopically labeled recombinant ligand‐binding domain of orphan nuclear receptor human B1‐binding factor/human liver receptor homologue 1 for NMR studies
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