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文献和实验Isolation and Culture of Mouse Germ Cells
cells have subsequently been found to be physiologically relevant to germ cell growth and development in vivo. For example, leukemia inhibitory factor (LIF) was identified as a potent germ cell survival factor and mitogen in culture (1 ). Subsequently
Cultures of Astroglia and Microglia from Primary Cultures of Mouse Neopallium
(see also Chapters 12 and 14 ). The medium can be modified either by changing its chemically defined components or by adding or deleting serum. It is also possible to add growth factors and/or cytokines, either to the culture medium in pure recombinant form
Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
growth factor inducing remodeling of epithelial cells. TGF-β1 induces a complex change of the gene expression profile, which leads to the induction of cell cycle arrest, increased cell migration, and spreading (2 �4 ). In general, determination
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