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文献和实验Fusion mediated by the human immunodeficiency virus type-1 (HIV-1) envelope (Env) glycoprotein and the cellular CD4/chemokine receptor complex is the first step in entry and is often analyzed in cell-cell fusion assays that require Env expression
Preparation of recombinant fibronectin fragments for functional and structural studies
integrin receptors. The mosaic nature of fibronectin permits it to be analyzed by a “dissection” strategy, where protein fragments generated by recombinant expression in E. coli , P. pastoris , and human cell lines are employed in structural and functional
Generation of Monoclonal Antibodies to Native Active Human Glycosyltransferases
Complex carbohydrates serve a wide range of biological functions in cells and tissues. Their biosynthesis involves more than 200 distinct glycosyltransferases in human cells, and the expression, properties, and topology of these enzymes
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