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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
2c + ++ Sources of information: Akerstrom, B., Brodin, T., Reis, K., and Bjorck, L. 1985. Protein G: A powerful tool for binding and detection of monoclonal and polyclonal antibodies. J. Immunol. 135:2589-2592. Akerstrom, B. and Bjorck, L. 1986
In Situ Immunofluorescence Analysis: Immunofluorescence Microscopy
cells (A) or after removal of cytoskeletal component (B) or in nuclear matrix preparations (C) . Run μ2 proteins were detected with a rabbit polyclonal Run μ2 antibody and an fluorescein isothiocyanate-conjugated antirabbit secondary antibody. DAPI
of an insoluble antibody-antigen complex. Rather, antibody-antigen complexes are removed from solution by addition of an insoluble form of an antibody binding protein such as Protein A, Protein G or second antibody (Figure 1). Thus, unlike other techniques based
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