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- CAU26518
- 2025年07月09日
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of X-gal can be easier to detect prior to carrying out the X-Phos reaction as background alkaline phosphatase staining can obscure it, particularly in whole mounts. Rinse the tissue very well with PBS prior to PLAP staining as residual ß-gal activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity 1. Obtain embryo samples, tube of AP substrate buffer and tube of phosphate buffered saline ( PBS ) for each group. Allow embryos
ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
antibody. 30 minutes. 5. Jet wash in tris/HC1 saline buffer pH 7.6. 6. Wash in buffer. 3 minutes. Steps 7-9 for POLYCLONAL Primary Antisera Only 7. Incubate in mouse anti-rabbit immunoglobulins. 30 minutes. 8. Jet wash in buffer.
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