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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
INTRODUCTION The formidable size and structure of polytene chromosomes allow mapping of chromosomal protein distributions at very high resolution. This protocol describes the preparation of polytene chromosomes from Drosophila larvae
CO-IMMUNOPRECIPITATION ANALYSIS OF PROTEIN-PROTEIN INTERACTIONS
for each protein. After 48 hours, harvest cells and wash with PBS -/- Transfer cells into 2 microfuge tubes and lyse each tube of cells with 1ml of: 20 mM Tris pH 7.5 100 mM NaCl 0.5% NP-40 0.5 mM EDTA 0.5 mM
Methods to Detect Protein Glutathionylation
) Primary antibody against protein of interest and corresponding species‐specific HRP‐conjugated secondary antibody, 1:1000 dilution in TBS‐T
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