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- 2025年07月08日
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文献和实验Generation of Poly(ADP-ribosyl)ation Deficient Mutants of the Transcription Factor, CTCF
to produce such mutants, demonstrated by the example of CTCF, a transcription factor. To achieve this in our study, the protein domain modified by PARylation was mapped and the amino acids, which can be potentially PARylated, selected. Mutations
Chromatin Immunoprecipitation (ChIP)
to be more problematic for monoclonal antibodies or peptide antibodies because these antibody preparations will usually recognize only a single epitope. Therefore, polyclonal antibodies generated against intact native proteins, fusion proteins, or large protein
Nanobody-Based Chromatin Immunoprecipitation
tailored to streamline ChIP. Here, we describe a Nanobody-based ChIP protocol which we have successfully used for genome-wide identification of the binding sites of the low-abundant transcription factor Ss-LrpB from the hyperthermoacidophilic
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