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文献和实验: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
). 9. Secondary antibody to use with goat polyclonal reagent: donkeyanti-goat IgG-horseradish peroxidase (HRP) (sc-2056;Santa Cruz Biotechnology). 10. Secondary antibody to use with rabbit polyclonal reagent: donkey anti-rabbit IgG-HRP (#NA934V, GE
itself was adapted from one obtained by Upstate. The immunohistochemistry procedure, adapted from Biomeda's manual, is for use of Biomeda's HistoScan kit based on a streptavidin-peroxidase/biotinylated second antibody detection system with 3-amino, 9-ethylcarbazole
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