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- 2025年07月12日
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
for mapping distributions of chromosome-associated proteins, identifying and characterizing cis -regulatory DNA elements bound by particular proteins, and mapping functional protein domains necessary for chromosomal binding or other activities
Chromatin Immunoprecipitation (ChIP)
to be more problematic for monoclonal antibodies or peptide antibodies because these antibody preparations will usually recognize only a single epitope. Therefore, polyclonal antibodies generated against intact native proteins, fusion proteins, or large protein
Strategies to Optimize Protein Expression in E. coli
characterization of the spine‐associated RapGAP (SPAR) PDZ domain. Protein Expr. Purif. 62:9‐14. Brown, B.L., Grigoriu, S., Kim, Y., Arruda, J.M., Davenport, A., Wood, T.K., Peti, W
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