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文献和实验Fluorescence Assays for Measuring Fatty Acid Binding and Transport Through Membranes
The authors’ laboratory has applied a series of different fluorescence assays for monitoring the binding and transport of fatty acids (FA) in model and biological membranes. The authors recently expanded their fluorescent assays
Detection of Lipid Hydroperoxide-Derived Protein Modification with Polyclonal Antibodies
. We also show the preparation of the polyclonal antibody to Nε -(hexanoyl)lysine, which is identified as a novel lipid-lysine adduct from the reaction mixture of linoleic acid hydroperoxide and lysine (5 ).
Epitope Mapping: Identiflcation of Antibody- Binding Sites on Protein Antigens
An epitope can be simply defined as that part of an antigen involved in its recogmtion by an antibody In the case of protein antigens, an epitope would consist of a group of mdlvidual ammo-acid side-chains close together on the protein surface
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