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- 规格:
250ug
Animal-Free Human IL-36γ (IL-1F9) (250ug)
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Product Details |
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Size |
250 µg |
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Species |
Human |
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Expression system |
E. coli |
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Amino acid sequence |
SMCKPITGTI NDLNQQVWTL QGQNLVAVPR SDSVTPVTVA VITCKYPEAL EQGRGDPIYL GIQNPEMCLY CEKVGEQPTL QLKEQKIMDL YGQPEPVKPF LFYRAKTGRT STLESVAFPD WFIASSKRDQ PIILTSELGK SYNTAFELNI ND |
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Molecular weight |
17 kDa |
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Class |
Recombinant |
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Type |
Protein |
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Purity |
95% by SDS-PAGE gel and HPLC analyses. |
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Endotoxin concentration |
<0.1 EU/µg |
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Activity |
Measured by its ability to induce IL-8 production by human PBMCs. |
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Conjugate |
Unconjugated |
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Form |
Lyophilized |
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Purification |
purified |
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Contains |
no preservative |
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Storage conditions |
-20°C |
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Applications |
Tested Dilution |
Publications |
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Western Blot (WB) |
Assay-dependent |
- |
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ELISA (ELISA) |
Assay-dependent |
- |
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Functional Assay (Functional) |
Assay-dependent |
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Product Specific Information
Recombinant Human IL-36gamma is a 17.0 kDa protein containing 152 amino acid residues.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot- specific Certificate of Analysis
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文献和实验Human Embryonic Stem (ES) Cell Protocols――Media and Reagents
Serum Free Media for human ES cells on MEFs: can last for 7-10 days Final Concentration Amount for 250ml Stock solution 80% DMEM-F12 200ml
PriCells: Methods of Isolation and Culture of Human Hepatocytes
. An aliquot of trypan blue solution (50 μL) should be mixed with the human hepatocyte suspension (250 μL) and allowed to stand for 2 min at room temperature. The cell suspension should then be analyzed using an improved Neubauer counting chamber
Intracellular Immunofluorescent Staining for Flow Cytometry
-γ mouse spleen ConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d) 2d/3d anti-CD3 (10ug/ml immobilized) + anti-CD28 (2ug/ml soluble) 5hr Brefeldin A XMG1.2 IL-1α mouse
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