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文献和实验Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
% confluent (see Fig. 1 ). REFERENCES McElroy S.L., Reijo Pera R.A. 2008a. Preparation of mouse fibroblast feeder cells for human embryonic stem cell culture. CSH Protocols (this issue). doi: 10.1101/pdb.prot5041.[Abstract/Free
and memory in aged mice via Fgf17. Nature (2022). 2. Castellano, J. M. et al. Human umbilical cord plasma proteins revitalize hippocampal function in aged mice. Nature 544, 488–492 (2017). 3. Fame, R. M. & Lehtinen, M. K. Emergence and developmental roles
Maintenance of Human Embryonic Stem Cells in Animal Serum- and Feeder Layer-Free Culture Conditions
and future clinical application require an animal-free and well-defined culture system for hESCs, these conventional conditions would prevent the use of hESCs in human therapy. This chapter describes optional culture conditions based on either animal-free
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