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- 2025年12月08日
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
Hapten Labeling of Nucleic Acids for Immuno-Polymerase Chain Reaction Applications
A method for the ultrasensitive protein detection in the range of 0.01 to 10,000 amol of the model antibody anti-mouse-IgG from rabbit is described, using a combination of Immunopolymerase chain reaction (PCR) and PCR-enzyme-linked
Immunoflourescence of Cultured Cells
as in 4). 7) Add secondary antibody as in 5) and 6) above. (e.g. FITC-conj goat anti-rabbit (1:1000) or Biotinylated isotype specific anti mouse for immunoperoxidase staining). Wash as before. 8) To minimize quenching of flourochrome mount
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