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- PeproTech
- 500-P120Bt-1MG
- 2026年02月05日
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
FLUORESCENCE IN SITU HYBRIDIZATION (FISH) ON HUMAN CHROMOSOMES AND INTERPHASE NUCLEI
-buffer at RT (a) or at 42°C (b) . incubate 20 min with 100µl/slide of 1:100 diluted biotinylated goat-anti-avidin (5% NFDM solution) + for FTCH 1:500 diluted mouse anti-digoxin (0.5% Blocking reagens solution) in moist in dark at RT
Hapten Labeling of Nucleic Acids for Immuno-Polymerase Chain Reaction Applications
A method for the ultrasensitive protein detection in the range of 0.01 to 10,000 amol of the model antibody anti-mouse-IgG from rabbit is described, using a combination of Immunopolymerase chain reaction (PCR) and PCR-enzyme-linked
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