- ￥56550
- PeproTech
- 500-P61Bt-1MG
- 2025年07月15日
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- 详细信息
- 技术资料
- 规格：
1mg
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Product Details |
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Size |
25 µg |
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Species Reactivity |
Mouse |
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Host/Isotype |
Rabbit |
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Class |
Polyclonal |
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Type |
Antibody |
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Conjugate |
Biotin |
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Immunogen |
E.coli-derived Recombinant Murine MCP-5 (CCL12) |
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Form |
Lyophilized |
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Concentration |
0.1-1.0 mg/mL |
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Purification |
Antigen affinity chromatography |
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Storage buffer |
PBS |
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Contains |
no preservative |
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Storage conditions |
-20°C |
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RRID |
AB_2930657 |
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Applications |
Tested Dilution |
Publications |
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Western Blot (WB) |
0.1-0.2 µg/mL |
- |
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ELISA (ELISA) |
0.25-1.0 µg/mL |
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Product Specific Information
AA Sequence of recombinant protein: GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MCP-5 (CCL12). Anti-Murine MCP-5 (CCL12)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mMCP-5 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg /mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MCP-5 (500-P61) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mMCP-5.
Western Blot: To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL.
Used in conjunction with compatible secondary reagents the detection limit for Recombinant mMCP-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P61BT-1MG will be provided as 2 x 500 µg
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MCP-5 Antibody (500-P61BT-25UG) in WB |
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Western Blot: To detect mMCP-5 by Western Blot analysis MCP-5 Polyclonal Antibody, Biotin (Product # 500-P61BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mMCP-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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MCP-5 Antibody (500-P61BT-25UG) in WB |
|
Western Blot: To detect mMCP-5 by Western Blot analysis MCP-5 PolyclonalAntibody, Biotin (Product # 500-P61BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL.Used in conjunction with compatible secondary reagents the detection limit for Recombinant mMCP-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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MCP-5 Antibody (500-P61BT-25UG) in ELISA |
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Sandwich ELISA: To detect mMCP-5 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of MCP-5 Polyclonal Antibody, Biotin (Product # 500-P61BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech MCP-5 Polyclonal Antibody (Product # 500-P61-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mMCP-5.
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