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- PeproTech
- 450-10-10UG
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
10ug
Human GDNF (10ug)
GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin,Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRa (a1-a4) receptors. GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons. Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101,which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine knot configuration.
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Product Details |
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Size |
10 µg |
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Species |
Human |
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Published Species |
Avian, Rat, Virus, Non-human primate, Amphibian, Hamster, Human, Mouse, Xenopus |
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Expression system |
E. coli |
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Amino acid sequence |
MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI |
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Molecular weight |
30.4 kDa |
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Class |
Recombinant |
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Type |
Protein |
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Purity |
98% by SDS-PAGE gel and HPLC analyses. |
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Endotoxin concentration |
<1 EU/µg |
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Activity |
Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml. |
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Conjugate |
Unconjugated |
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Form |
Lyophilized |
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Purification |
purified |
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Contains |
no preservative |
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Storage conditions |
-20°C |
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Applications |
Tested Dilution |
Publications |
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Western Blot (WB) |
Assay-dependent |
1 Publication |
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ELISA (ELISA) |
Assay-dependent |
- |
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Functional Assay (Functional) |
Assay-dependent |
2 Publications |
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In vitro Assay (IV) |
- |
285 Publications |
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Miscellaneous PubMed (Misc) |
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49 Publications |
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Product Specific Information
The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called
monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain
disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot- specific Certificate of Analysis
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Human GDNF Protein (450-10-10UG) in Functional
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| Bioassay analysis of Human GDNF Recombinant Protein, PeproTech® (Product # 450-10-1MG). | |
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Human GDNF Protein (450-10-10UG) in Functional |
| WHO Comparison of Human GDNF Recombinant Protein, PeproTech® (Product # 450-10-1MG). |
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文献和实验[1]Sato T, Aiyama Y, Ishii-Inagaki M, Hara K, Tsunekawa N, Harikae K, Uemura-Kamata M, Shinomura M, Zhu XB, Maeda S, Kuwahara-Otani S, Kudo A, Kawakami H, Kanai-Azuma M, Fujiwara M, Miyamae Y, Yoshida S, Seki M, Kurohmaru M, Kanai Y. Cyclical and patch-like GDNF distribution along the basal surface of Sertoli cells in mouse and hamster testes. PLoS One. 2011;6(12):e28367. doi: 10.1371/journal.pone.0028367. Epub 2011 Dec 9. PMID: 22174794; PMCID: PMC3235125.
[2]Bollmann C, Schöning S, Kotschnew K, Grosse J, Heitzig N, Fischer von Mollard G. Primary neurons lacking the SNAREs vti1a and vti1b show altered neuronal development. Neural Dev. 2022 Nov 22;17(1):12. doi: 10.1186/s13064-022-00168-2. PMID: 36419086; PMCID: PMC9682837.
[3]Renko JM, Mahato AK, Visnapuu T, Valkonen K, Karelson M, Voutilainen MH, Saarma M, Tuominen RK, Sidorova YA. Neuroprotective Potential of a Small Molecule RET Agonist in Cultured Dopamine Neurons and Hemiparkinsonian Rats. J Parkinsons Dis. 2021;11(3):1023-1046. doi: 10.3233/JPD-202400. PMID: 34024778; PMCID: PMC8461720.
[4]Wen MH, Barbosa Triana H, Butler R, Hu HW, Dai YH, Lawrence N, Hong JJ, Garrett N, Jones-Green R, Rawlins EL, Dong Z, Koziol MJ, Gurdon JB. Deterministic nuclear reprogramming of mammalian nuclei to a totipotency-like state by Amphibian meiotic oocytes for stem cell therapy in humans. Biol Open. 2024 Mar 1;13(3):bio060011. doi: 10.1242/bio.060011. Epub 2024 Mar 1. PMID: 37982514; PMCID: PMC10924218.
[5]Fan Y, Huang S, Li S, Wu B, Zhao Q, Huang L, Zheng Z, Xie X, Liu J, Huang W, Sun J, Zhu X, Zhu J, Xiang AP, Li W. The adipose-neural axis is involved in epicardial adipose tissue-related cardiac arrhythmias. Cell Rep Med. 2024 May 21;5(5):101559. doi: 10.1016/j.xcrm.2024.101559. Epub 2024 May 13. PMID: 38744275; PMCID: PMC11148799.
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