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文献和实验Materials: P3X63Ag8.653 murine myeloma or YB2/0 (maintained at 50 w/v PEG 1500, warmed to 37° C Medium: IMDM supplemented with 20 fetal bovine serum, 4 mM L-glutamine, 1 mM sodium pyruvate, 50 U penicillin
General Fusion Protocol Materials: P3X63Ag8.653 murine myeloma or YB2/0 (maintained at 6 /ml) 50% w/v PEG 1500, warmed to 37° C Medium: IMDM supplemented with 20% fetal bovine serum, 4 mM L-
Characterization of Chemokine Receptors
is also described which also uses SPA methodology to measure GTP?gamma?S binding to chemokine receptors in cell membranes. The two basic protocols can be used to determine the binding of compounds to chemokine receptors, while the alternate protocol determines
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