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- 技术资料
- 保质期:
5年
- 供应商:
山东绿都
- 保存条件:
常温
- 规格:
1mg
英文名称 Cholera toxin from uibrio cholerae;Choleragen;Choleragenoid
CAS识别号 9012-63-9
MDL识别号 Null,without related properties.
EC识别号 Null,without related properties.
Beilstein Null,without related properties.
分子式 Null,without related properties.
分子量 Null,without related properties.
| 分子结构图 |
质量标准 蛋白:~5% Lowry-TCA
活力:1×105~1×106 units protein
活力定义:One unit increases adenylate cyclase activity 6-fold, measured as [3H]-cAMP present, using approx. 70,000 CHO cells after incubation for 90 min at 37 °C with [3H]-adenine followed by a 15 min incubation with 1 mM 3-isobutyl-1-mthylxanthine (IBMX).
Physical form:Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTA。Toxin consisting of an A subunit (27 kDa) surrounded by five B subunits (approximately 12 kDa each), which attach the toxin to ganglioside GM1 on the cell surface. The A subunit catalyzes ADP-ribosylation of the α-subunit of the stimulatory G protein (Gαs), reducing GTPase activity and activating the α-subunit. This activation of Gαs leads to an increase in the activity of adenylyl cyclase, resulting in increased levels of cAMP. Also ADP-ribosylates transducin in the eye rod outer segments, inactivating its GTPase activity. Cholera toxin has also been reported to ADP-ribosylate tubulin. Shown to be a potent mucosal vaccine adjuvant, inducing T helper cell type 2 responses by inhibiting the production of interleukin-12.
储存条件 常温密封避光保存,Stored in cool, dry and airproof.
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文献和实验素,对神经组织有高度选择性,在神经肌肉接头与神经节苷受体结合后,可沿神经元轴突逆行而上,最后到达脊髓中的抑制性GABA神经元轴突而抑制神经递质的释放,从而引起破伤风所特有的痉挛。 (三)霍乱毒素和大肠埃希菌不耐热毒素(LT) 两种毒素的结构和作用机制基本相同,都是通过激活细胞腺苷酸环化酶,增加cAMP含量而促进氯化物和水的分泌。此外,前列腺素和其他递质的产生也可能参与此过程。 (四)大肠埃希菌耐热毒素(ST)
/578 7)cy3 490/530 8)cy5 650/690 (1)细胞表面抗原、胞内某种蛋白 免役荧光标记: 与抗体耦联, 直标: 一抗+荧光探针 间标: 二抗+荧光探针 如: 微管蛋白 抗 tublin抗体+荧光探针 肌动蛋白 Palloidine+荧光探针 (2)细胞膜表面受体 配体+荧光探针 如: nAchR、mAchR、多巴胺受体(D1,D2) 标记 Gm1: 霍乱毒素受体 霍乱毒素
霍乱毒素 CT-HRP cholera-toxin-horseradish peroxidase 霍乱毒素辣根过氧化物酶 CUC Chronic ulcerous colonitis 慢性溃疡性结肠炎 CVD cerebrovascular disease 脑血管病 DA dopamine 多巴胺 DAB diaminobezidin 二氨基联苯氨 DBA Dolichos bifows agglutinin 双花扁豆凝集素 DBH
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