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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液体
- 适应物种:
Human;Mouse;Rat
- 保质期:
1年
- 库存:
100
- 供应商:
ImmunoWay Biotechnology Company
- 宿主:
Rabbit
- 应用范围:
WB
- 规格:
40μ/100ul/200ul
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文献和实验ELISA PROTOCOL 【Yale University 】
1 hour at room temperature.4) Discard the primary antibody solution and wash 3X with TST.5) Add 50 µl of diluted secondary antibody conjugated to AlkalinePhosphatase. I use a 1:500 dilution of rabbit anti-mouse IgG
ELISA PROTOCOL(Yale University)
1. Materials (1) TS Buffer: 10 mM Tris pH 8.0 150 mM NaCl (2) TST Buffer: TS Buffer with 0.05% Tween-20 (3) Alkaline Phosphatase Buffer: 50 mM NaCarbonate 1 mM MgCl2 (Bring to pH 9.8 with 1 N HCl) (4) AP Substrate: p-nitrophenyl phosphate (5 mg
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
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