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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液体
- 适应物种:
Human;Mouse
- 保质期:
1年
- 库存:
100
- 供应商:
ImmunoWay Biotechnology Company
- 宿主:
Rabbit
- 应用范围:
WB;ELISA
- 规格:
40μ/100ul/200ul
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文献和实验Myosin Light Chain Preparation
in lyophilizer bottles and freeze in liquid nitrogen.Put on lyophilizer and leave until dry (overnight or over weekend). Day 5 22.Resuspend separate light chain pools 1 and 2 in a small volume of Dialyzing Solution G.Pipette into dialyzing tubing (size #20
Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
Generation of Rabbit Immune Libraries
for the combinatorial cloning of the variable heavy and light chain immunoglobulin repertoire of rabbits for the subsequent phage display of rabbit scFv antibody fragments is described.
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