- 询价
- Immunoway
- YC0069
- 2025年07月13日
- IF;WB;IHC;ELISA
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液体
- 适应物种：
Human;Mouse;Rat
- 保质期：
1年
- 库存：
100
- 供应商：
ImmunoWay Biotechnology Company
- 宿主：
Rabbit
- 应用范围：
IF;WB;IHC;ELISA
- 规格：
40μ/100ul/200ul
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文献和实验Neoepitope Antibodies Against MMP-Cleaved and Aggrecanase-Cleaved Aggrecan
as examples: the monoclonal anti-FFGVG antibody (AF-28) and the polyclonal anti-DIPEN antisera.
Polyclonal and Monoclonal Antibodies
Polyclonal Antibody Immunization of Mice: Injections should be made at intervals of at least two weeks.. We have been using either of the two following adjuvants with success: Freund adjuvant and MPL+TDM adjuvant ( RIBI ImmunoChem
(either undiluted or 1:1 with PBT) or diluted polyclonal antiserum and incubate at 4 C overnight 8. Rinse with PBT 3 times, then wash 4 times for 15 minutes each 9. Add secondary antibody (usually 1:200 in PBT) and incubate 2 hours at room temperature
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