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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Mouse, Monoclonal/IgG1, Kappa
- 形态:
液体
- 保存条件:
2-8℃
- 克隆性:
单克隆
- 适应物种:
Human; Mouse (predicted: Rat)
- 保质期:
一年
- 级别:
病理级
- 库存:
100
- 供应商:
ImmunoWay Biotechnology Company
- 宿主:
小鼠
- 应用范围:
IHC
- 规格:
3ml/6ml/10ml
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文献和实验Cleavage of Plasmid DNA by Eukaryotic Topoisomerase II
Topoisomerase II is an essential enzyme that is required for a number of critical nuclear processes. All of the catalytic functions of topoisomerase II require the enzyme to generate a transient double-stranded break in the backbone
The Dynamics of DNA Topoisomerase II in Living Cells
Here, we describe methods to prepare a mammalian expression plasmid encoding EGFP fused to the amino-terminus of human DNA topoisomerase IIα (Topo IIα) for use in studying the dynamics of Topo IIα in living cells. In previous studies
Use of a Real-Time, Coupled Assay to Measure the ATPase Activity of DNA Topoisomerase II
This chapter describes the use of a common spectrophotometric assay for following the rate of ATP hydrolysis as applied to type II DNA topoisomerases. It is called a “coupled assay,” because each time an ATP molecule is hydro-lyzed
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